Determination of Ceruloplasmin in Human Serum by Immunoaffinity Chromatography and Size-Exclusion Chromatography-ICP-MS
نویسنده
چکیده
A new method is described for the analysis of copper (Cu) containing ceruloplasmin (Cp) in human serum. The method uses immunoaffinity plus size-exclusion chromatography (SEC) to separate Cp from other proteins and from inorganic ions, with inductively coupled plasma mass spectrometry (ICP-MS) detection of 63Cu and 65Cu to identify the protein. A reference serum certified for Cp was used to establish method accuracy and precision. The method was applied to 47 human sera samples with one of four different diseases plus a set of normal controls. The Cp concentration was correlated with the total Cu concentration measured by direct ICP-MS analysis. The results demonstrated that SEC-ICP-MS can accurately and reproducibly measure Cp in human serum that is depleted of six most abundant proteins. Determination of Ceruloplasmin in Human Serum by Immunoaffinity Chromatography and Size-Exclusion Chromatography-ICP-MS
منابع مشابه
Determination of ceruloplasmin in human serum by SEC-ICPMS.
This paper describes an analytical method for the determination of ceruloplasmin (Cp) in human serum. The method uses immunoaffinity chromatography and size-exclusion chromatography (SEC) to "purify" the serum sample prior to analysis of 63Cu and 65Cu by inductively-coupled plasma mass spectrometry (ICPMS). By removing the six most abundant proteins from serum with immunoaffinity chromatography...
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